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KMID : 0379119920200030222
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Korean Journal of Mycology
1992 Volume.20 No. 3 p.222 ~ p.228
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Characteristics of Transformants in Pleurotus florida
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김경수/Kim, Kyung Soo
차동열/변명옥/Cha, Dong Yeul/Byun, Myung Ok
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Abstract
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Pleurotus florida was transformed by complementation of auxotrophic mutant using chimeric plasmid containing Flammulina velutipes leu 2 gene and pBR 322 replicon. Mycelial morphology of transformants was grown and compared on mushroom complete and minimal median. Transformants were mated with monokaryoo and their genetic recombination was investigated for the morphology of fruitbody and spore analysis. Lea^+ transformants showed same mating type of A₁B₁ as to the untransformed mutant. The transformant and the untransformed mutant were mated with monokaryon of which mating type is A₂B₁, respectively. Although fruitbody of the untransformed mutant was not produced, leu^+ transformant produced fruitbody. Spore analysis showed that leucine requiring spores from frutibody of leu^+ transformant were diminished when compared with those of untransformed mutant.
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